Because of this, probe labeled with FAM perform best in cultured cells. This auto-fluorescence can make it difficult to discern true RNA transcripts from background, and is especially true in tissue where auto-fluorescence is generally already higher. Special Notes and Considerations: The green channel where FAM fluoresces is not ideal for RNA FISH as there tends to be high auto-fluorescence from the specimen itself.
FAM fluoresces in the green region of the visible spectrum.
General Description: The Fluorescein modification incorporates a fluorescein (FAM) moiety at the terminus of an oligonucleotide.
